Eman A. El-Ashiry,

ABSTRACT

Objectives: To Assess The Curative Capability Of Adipose Tissue Derived Mesenchymal Stem Cells ?AT-MSCs? Contrasted With Bone Marrow Derived Mesenchymal Stem Cells ?BM-MSCs? On Healing Of Surgically Formed Cleft Lip In Dogs.

Materials and Methods: Split Mouth Experimental Study Was Done On 12 Healthy And Vaccinated Mongrel Dogs, 12-18 Months And Weight Range Of 20kgs. Dogs Were Divided Into 2 Groups: In -group A, The Test Side, The Surgically Created Cleft Lip On The Right Side Of Maxilla Was Transplanted With ?AT-MSCs? And Scaffold As Well As Growth Factors. While, In The Control Side, The Surgically Created Cleft Lip On The Left Side Of Maxilla Was Filled With Scaffold And Growth Factors Only. In-group B, The Test Side, The Surgically Created Cleft Lip On The Right Side Of Maxilla Was Transplanted With ?BM-MSCs? And Scaffold As Well As Growth Factors. The Control Side, The Surgically Created Cleft Lip On The Left Side Of Maxilla Was Transplanted With Scaffold And Growth Factors Only. Resorbable Sutures Were Used To Suture The Flaps. The Collected Samples Were Evaluated Clinically After 1.5- And 3-months Following Scarification Of The Dogs. The Data Was Analysed Using T-test And Descriptive Procedure (p=0.05).

Results: ?AT-MSCs? As Well As ?BM-MSCs? Are A Valuable Source For Soft Tissue Healing. Both Secrete Healing Markers ?VEGF, Collagen, TGF, And ALP? That Paly Significant Role In Restoring Soft Tissue Integrity. ?AT-MSCs And BM-MSCs? Effects Were Documented Clinically And Immunohistochemically, As There Were Areas Of New Vascularization That Assure Great Potentiality Toward Healing. ?AT-MSCs? Or ?BM-MSCs? Increase Regeneration And Healing Of The Defects As Early As 1.5 And 3 Months. Conclusion: AT-MSCs And BM-MSCs Are Interesting Tools In Soft Tissue Healing. ?AT-MSCs? In Experimental Investigations Revealed Comparable Results To ?BM-MSCs?. Also, It Is Easier In Harvesting, Requires A Secure Method To Get Stem Cells As Well As Lesser Chance Of Infection, And Lower Cost.